A Fast Food Diet and Exercise Led to a Disrupted and Pathogenic Methylome in Mouse Liver
GSE85772 · GEO
TL;DR — A 523.3 MB bulk RNA-seq deposit (GSE85772, 2 file(s), liver) in the MASLD / NAFLD / NASH data lake. large (15.2 GB).
Biological premise.
This deposit is included in the MASLD (metabolic dysfunction-associated steatotic liver disease) data lake for the following curated reason: large (15.2 GB). No free-text abstract was exposed by the repository API; the measured file inventory below is authoritative for what the deposit contains.
Every file in this dataset is a bulk RNA-seq readout relevant to MASLD / non-alcoholic fatty liver disease (NAFLD) / non-alcoholic steatohepatitis (NASH), landed from GEO.
Assay and processing.
Modality: bulk RNA-seq (repository subtype: bulk-RNAseq). Tier-1 (must-have) for this modality = raw/normalized gene × sample expression matrix + per-sample design; Tier-2 (recommended) = author DE tables, design/contrasts, gene annotation; Tier-3 (optional / raw) = raw reads (FASTQ/BAM in SRA/ENA), reference build + quantifier version. The Completeness line in each dataset block below states, measured from the landed files, which tiers are present.
Sources.
| Disease | MASLD / NAFLD / NASH |
| Assay / modality | bulk RNA-seq — bulk-RNAseq |
| Organisms | unspecified |
| Repository | GEO — NCBI GEO FTP |
| Accession | GSE85772 |
| Access | Public (open); files mirrored in this dataset's input/ folder. |
| Number of studies | 1 |
| Number of files | 2 |
| Total size | 523,297,301 bytes (≈ 523.3 MB) |
Landed sources at a glance.
| Source | Access | Files |
|---|---|---|
| GEO | public / OPEN-processed | 2 |
The deposit at a glance.
| Accession | Description | Organism | Samples | Scale |
|---|---|---|---|---|
| GSE85772 | large (15.2 GB) | unspecified | n/a | 2 files · 523.3 MB |
GEO record · 2 files · 523.3 MB
Sample source. unspecified material; MASLD/NAFLD/NASH context (large (15.2 GB)).
Samples and retention. Sample count not declared in the repository index; see the per-sample metadata file(s) listed below.
Experiment design. Repository subtype: bulk-RNAseq; disease context MASLD/NAFLD/NASH.
Metadata available. See the annotated file table below; per-sample metadata ships in the ISA-Tab / SDRF / metadata file(s) where present.
Files (annotated).
| File | What it is |
|---|---|
GSE85772_RAW.tar | GEO per-sample raw supplementary bundle (tar of per-GSM files) |
filelist.txt | Text data / metadata table (35 data rows × 5 cols) |
How this experiment became a dataset. GSE85772 resolved via the GEO record API → 2 open file(s) fetched into this dataset's input/ folder → sizes verified on disk (523,297,301 bytes (≈ 523.3 MB)).
Completeness (bulk RNA-seq). No canonical Tier-1 slot auto-detected among the landed files. Not detected among landed files: expression matrix (Tier-1); per-sample design (Tier-1). (Tiers per the matching modality skill; assessment measured from landed filenames/headers.)
GSE85772_RAW.tar (expression matrix) to run DESeq2/edgeR or limma contrasts and MASLD signatures.Reproduce. All files are in this dataset's input/ folder; the source of record is GSE85772 (https://ftp.ncbi.nlm.nih.gov/geo/series/GSE85nnn/GSE85772/suppl/). Re-fetch with the MASLD crawler (masld_download_pass.py --only GSE85772); sizes are verified on disk and recorded in this dataset's metadata.json.
A Fast Food Diet and Exercise Led to a Disrupted and Pathogenic Methylome in Mouse Liver. GEO accession GSE85772. https://ftp.ncbi.nlm.nih.gov/geo/series/GSE85nnn/GSE85772/suppl/